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Image Search Results
Journal:
Article Title: Role of interleukin 15 in colitis induced by dextran sulphate sodium in mice
doi: 10.1136/gut.2005.076000
Figure Lengend Snippet: Figure 5 Effects of in vivo treatment with anti‐CD8 monoclonal antibody (mAb) and asialo‐GM1 Abs on dextran sulphate sodium (DSS) induced colitis in C57BL/6 mice. (A) For in vivo cell depletion, both anti‐CD8 mAb and anti‐asialo GM1 Ab or isotype control IgG was injected intraperitoneally into C57BL/6 mice once a week from two days before administration of DSS. Survival rates of these mice administered 2% DSS were monitored every day (n = 5 per group). (B–D) Lamina propria (LP) cells from anti‐CD8 and asialo‐GM1 treated mice or control IgG treated mice on day 5 after DSS administration or naïve C57BL/6 mice (DSS(−)) were prepared and cultured without stimulation for 24 hours at 37°C. Tumour necrosis factor (TNF)‐α (B), interferon (IFN)‐γ (C), and IL‐12p40 (D) concentrations in culture supernatants of LP cells were determined by enzyme linked immunosorbent assay (n = 4 per group). Statistically significant differences are shown. ND, not detectable.
Article Snippet: Cytokine activity was assayed by enzyme linked immunosorbent assay (ELISA) using an ELISA Development Kit for
Techniques: In Vivo, Control, Injection, Cell Culture, Enzyme-linked Immunosorbent Assay
Journal:
Article Title: Role of interleukin 15 in colitis induced by dextran sulphate sodium in mice
doi: 10.1136/gut.2005.076000
Figure Lengend Snippet: Figure 6 Dextran sulphate sodium (DSS) induced chronic colitis in interleukin (IL)‐15 knockout (KO) mice. IL‐15 KO mice and wild‐type (WT) mice were administered 2% DSS on days 0–5, 10–15, and 20–25. Body weight (A) and disease activity index (B) were monitored every day and values for body weight are expressed as percentage of body weight on day 0. Each point represents the mean (SD) (n = 7 per group). (C–E) Lamina propria (LP) cells from IL‐15 KO mice or WT mice on day 30 after DSS administration or naïve IL‐15 KO mice or WT mice (DSS(−)) were prepared and cultured without stimulation for 24 hours at 37°C. Production of tumour necrosis factor (TNF)‐α (C), interferon (IFN)‐γ (D), or IL‐12p40 (E) in culture supernatants of LP cells was determined by enzyme linked immunosorbent assay (n = 4 per group). Statistically significant differences are shown: * p<0.05. ND, not detectable.
Article Snippet: Cytokine activity was assayed by enzyme linked immunosorbent assay (ELISA) using an ELISA Development Kit for
Techniques: Knock-Out, Activity Assay, Cell Culture, Enzyme-linked Immunosorbent Assay